Agricultural Science

Agricultural Science

ISSN: 2291-4471 (Print)    ISSN: 2291-448X (Online)

Volume 4 (2016), No. 3, Pages 6-12

DOI: 10.12735/as.v4n3p06

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Short-Term Liquid Nitrogen Storage of Maize, Common Bean and Soybean Seeds Modifies Their Biochemical Composition

Melissa Arguedas1  Aurora Perez2  Ana Abdelnour1  Martha Hernandez2  Florent Engelmann3  Marcos Edel Martínez2  Lourdes Yabor2  José Carlos Lorenzo2 

1Escuela de Biología, Instituto Tecnológico de Costa Rica, Cartago, Costa Rica
2Laboratory for Plant Breeding, Centro de Bioplantas, Universidad de Ciego de Avila, Ciego de Avila 69450, Cuba
3IRD, UMR DIADE, France

URL: http://dx.doi.org/10.12735/as.v4n3p06

To Cite this Article     Article Views: 723     Downloads: 282  Since deposited on 2016-08-12

Abstract

We studied the effects of liquid nitrogen storage of maize, common bean and soybean seeds on their germination, electrolyte leakage, levels of chlorophylls, phenolics, aldehydes, proteins and peroxidase activity. After storage for 28 days, seeds were retrieved from liquid nitrogen, some were set to germinate and others were analyzed biochemically. No phenotypic modifications were observed visually 5 days after beginning of germination, although percentage of seed germination was reduced by LN in maize and soybean. Moreover, numerous significant effects of seed cryopreservation were recorded at the biochemical status. In maize seeds, the most important and statistically significant modifications were observed in the increased levels of chlorophyll b and total chlorophyll pigments and in the decreased contents of free phenolics after 28 days of exposure to LN, compared to the control treatment. In common bean, relevant changes were observed in the increased electrolyte leakage and in the reduced levels of chlorophyll pigments (b, total) and free phenolics. In soybean, modifications were observed in the increased levels of chlorophyll pigments (a, b, total), malondialdehyde and electrolyte leakage, and in the decreased peroxidase activity. We have shown for the first time that immersion of maize, common bean and soybean seeds in liquid nitrogen modified the levels of different biochemicals.

Keywords: Zea mays; Phaseolus vulgaris; Glycine max; cryostorage; phenotypic variation

To Cite this Article: Arguedas, M., Pérez, A., Abdelnour, A., Hernández, M., Engelmann, F., Martínez, M. E., Yabor, L., & Lorenzo, J. C. (2016). Short-term liquid nitrogen storage of maize, common bean and soybean seeds modifies their biochemical composition. Agricultural Science, 4(3), 6-12. http://dx.doi.org/10.12735/as.v4n3p06

Copyright © M. Arguedas et al.

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This article is published under license to Science and Education Centre of North America. This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International License.

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Short-Term Liquid Nitrogen Storage of Maize, Common Bean and Soybean Seeds Modifies Their Biochemical Composition
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