Plant Science International

Plant Science International

ISSN: 2368-8181 (Print)    ISSN: 2368-819X (Online)

Volume 2 (2015), No. 1, Pages 1-12

DOI: 10.12735/psi.v2n1p01

Download Full Text:

Micropropagation of Yeheb from Shoot Tip: An Endangered Multipurpose Shrub

Chanie Derso1  2  Tileye Feyissa2 

1Department of Dry land Crop Science, Jigjiga University, Jigjiga, Ethiopia
2Institute of Biotechnology, Addis Ababa University, Addis Ababa, Ethiopia

URL: http://dx.doi.org/10.12735/psi.v2n1p01

To Cite this Article     Article Views: 406     Downloads: 310  Since deposited on 2015-12-08

Abstract

The study focuses on in vitro propagation of an endangered species of C. edulis. In vitro propagation has advantages to address low seed viability and over exploitation problems. Thus, the objective of this study is to develop micropropagation method from shoot tip explant. To do so, seeds were de-coated and disinfected using 5% (v/v) Clorox and cultured on growth regulators-free solid MS medium. Shoots were initiated on MS medium containing BAP alone or in combination with IBA. Then, initiated shoots were multiplied on solid MS medium supplemented with various concentration and combinations of BAP and TDZ alone and in combination with IBA or NAA respectively. Shootlets were cultured on full, half and one-third strength MS medium containing 0.5, 1.0, 1.5, 2.0 and 2.5 mg/l IBA and NAA for root induction. As a preliminary test for rooting, B5 and WPM media, both supplemented with 0.5, 1.0, 2.0 mg/l IBA, were used. Shootlets were cultured on full strength MS medium containing higher concentration of 3.0 and 5.0 mg/l IBA and 1.0 g/l activated charcoal for four days and then transferred into growth regulators-free MS medium for a month for root induction as preliminary test. The highest percentage of shoot induction (70.8%) was obtained on medium containing 0.5 mg/l BAP in combination with 0.01 mg/l IBA. The highest number of shoots (4.2 ± 0.85) produced per explant was achieved on MS multiplication medium containing 2.0 mg/l BAP. None of the shoots induced roots in any of different MS salt strengths and other media. As conclusion, this is the first try of micropropagation and the existing result is valuable for cryopreservation of this endangered plant.

Keywords: Cordeauxia edulis, in vitro; micropropagation, plant growth regulators, shoot multiplication, shoot tip culture

To Cite this Article: Derso, C., & Feyissa, T. (2015). Micropropagation of yeheb from shoot tip: An endangered multipurpose shrub. Plant Science International, 2(1), 1-12. http://dx.doi.org/10.12735/psi.v2n1p01

Copyright © Chanie Derso & Tileye Feyissa

Creative Commons License
This article is published under license to Science and Education Centre of North America. This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International License.

To make sure that you can receive messages from us, please add the 'todayscience.org' domain to your e-mail 'safe list'. If you do not receive e-mail in your 'inbox', check your 'bulk mail' or 'junk mail' folders.

Micropropagation of Yeheb from Shoot Tip: An Endangered Multipurpose Shrub
reviwers